CD11b antibody

Catalog No. ABIN319553

The Rat Monoclonal anti-CD11b antibody has been validated for FACS, IP, IHC (fro), Func and IHC (p). It is suitable to detect CD11b in samples from Human, Mouse and Rabbit.

Quick Overview for CD11b antibody (ABIN319553)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rabbit

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: M1-70-15

Product Details anti-CD11b Antibody

Cross-Reactivity (Details): Species reactivity (expected):Human, Rabbit.
Species reactivity (tested):Mouse.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Endotoxin Level: Low Endotoxin

Immunogen: T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Immunoprecipitation. Flow Cytometry: 1/50 - 1/100 (routinely tested in flow cytometry on mouse peritoneal macrophages). Functional Assays: Clone M1/70.15 has been reported to block iC3b binding to its receptor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level < 0.01EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 10090

UniProt: P05555

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation